?-Hydroxysterol distribution as determined by freeze-fracture cytochemistry
نویسندگان
چکیده
منابع مشابه
Freeze-fracture cytochemistry in cell biology.
The term freeze-fracture cytochemistry embraces a series of techniques which share the goal of chemical identification of the structural components viewed in freeze-fracture replicas. As one of the major features of freeze fracture is its ability to provide planar views of membranes, a major emphasis in freeze-fracture cytochemistry is to identify integral membrane proteins, study their spatial...
متن کاملThe cell surface of Trypanosoma cruzi: cytochemistry and freeze-fracture.
The ultrastructure of epimastigotes of Trypanosoma cruzi, obtained from acellular cultures, and bloodstream trypomastigotes, isolated from infected mice, were studied by thin-sectioning and freeze-fracturing techniques. Epimastigotes showed a thin (5 nm) surface coat when stained with ruthenium red, while the surface coat of trypomastigotes was more prominent (15 nm thick). Both P and E faces o...
متن کاملFreeze-fracture cytochemistry: partition of glycophorin in freeze- fractured human erythrocyte membranes
Thin-section and critical-point-dried fracture-labeled preparations are used to determine the distribution and partition of glycophorin-associated wheat germ agglutinin (WGA) binding sites over protoplasmic and exoplasmic faces of freeze-fractured human erythrocyte membranes. Most wheat germ agglutinin binding sites are found over exoplasmic faces. Label is sparse over the protoplasmic faces. T...
متن کاملFreeze-fracture cytochemistry: a new fracture-labeling method for topological analysis of biomembrane molecules.
Freeze-fracture cytochemistry allows visualization of cellular and molecular characteristics of biomembranes in situ. In this review, we discuss freeze-fracture cytochemistry with special reference to a new cytochemical labeling of replicas, the detergent-digestion fracture-labeling technique. In this procedure, unfixed cells are rapidly-frozen, freeze-fractured, and physically stabilized by ev...
متن کاملTransmembrane phospholipid distribution revealed by freeze-fracture replica labeling.
We propose the use of membrane splitting by freeze-fracture for differential phospholipid analysis of protoplasmic and exoplasmic membrane leaflets (halves). Unfixed cells or tissues are quick-frozen, freeze-fractured, and platinum-carbon (Pt/C) shadowed. The Pt/C replicas are then treated with 2.5% sodium dodecyl sulfate (SDS) to solubilize unfractured membranes and to release cytoplasm or con...
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ژورنال
عنوان ژورنال: The Histochemical Journal
سال: 1981
ISSN: 0018-2214,1573-6865
DOI: 10.1007/bf01002709